Journal: STAR Protocols

Article Title: Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing

doi: 10.1016/j.xpro.2025.104296

Figure Lengend Snippet: 3D light sheet and 2D multi-cyclic imaging data comparison (Mouse Glioblastoma) (A) Imaris 3D surface rendering of autofluorescence (cyan) and glioblastoma target cells stained with anti-GFP-Alexa Fluor 647 nanobody (red). (B) Imaris 3D surface rendering of autofluorescence (cyan) and glioblastoma target cells stained with anti-GFP-Alexa Fluor 647 nanobody (red) with target plane in yellow. (C) Optical section of target plane of interest. (D) Fluorescence image of physical cryosection. (E) MICS image of section shown in D. (F) MICS image indicating anti-GFP-Alexa Fluor 647 nanobody (red) staining. (G) Magnified merged four color multiparameter MICS image with anti-EGFR (magenta), anti-GFAP (green), anti-NeuN (blue), anti-CD146 (yellow). (H–P) Nine exemplary MICS images with merges of anti-GFP-Alexa Fluor 647 nanobody staining (red) and antibody-conjugates against EGFR (H), Neurofilament (I), Nestin (J), GFAP (K), CD44 (L), CD146 (M), NeuN (N), EphA2 (O) and GLAST (P) (gray) (see “Antibodies”). Scale bars: (A–F) 500 μm; (G) 50 μm; (H–P) 500 μm.

Article Snippet: EphA2 antibody, anti-mouse, APC, REAfinity , Miltenyi Biotec B.V. & Co. KG , Cat# 130-109-187 RRID: AB_2651638.

Techniques: Imaging, Comparison, Staining, Fluorescence

Journal: bioRxiv

Article Title: Skeletal Metastasis of Prostate Cancer Is Augmented by Activation of EphA2 Noncanonical Signaling and Ligand-Deficient Bone Microenvironment

doi: 10.1101/2024.09.25.615079

Figure Lengend Snippet: A) Analysis of log 2 transformed FPKM gene expression values extracted from bulk RNA seq published previously (Smith R. et al. Scientific Reports 2020 ) shows high EphA2 expression in castration resistant, commercially available human PCa cell lines. B) Immunoblot characterization of select human PCa cell lines show increased EphA2 expression and S897 phosphorylation in mCRPC derived cell lines. C) EphA2 expression in representative human prostate cancer cell lines. D) Lysate of human prostate cancer tissues reveal increased expression of EphA2 in bone metastasis samples. Lysates were prepared from micro-dissected human prostate cancer tissues under the Rapid Autopsy Program at the Univ. Mich. PCa SPORE.

Article Snippet: Other antibodies were purchased as follows: Rabbit monoclonal anti-GAPDH (Cell Signaling Technology #2118S), rabbit monoclonal anti-pS473-Akt (Cell Signaling Technology #4060), rabbit polyclonal anti-pErk1/pErk2 (Cell Signaling Technology #9101S), rabbit monoclonal anti-EphA2 (Cell Signaling Technology #6697S), rat monoclonal anti-EphA2-PE (R&D Systems FAB639P), rabbit monoclonal anti-AR (Abcam ab133273), rabbit polyclonal anti-pY772-EphA2 (Cell Signaling Technology #8244S), rabbit monoclonal anti-pY588-EphA2 (Cell Signaling Technology #12677S), mouse monoclonal anti-Tubulin (R&D Systems MAB9344) Goat anti-rabbit and goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and from Biorad (#1706515, and #1706516 respectively).

Techniques: Transformation Assay, Expressing, RNA Sequencing Assay, Western Blot, Derivative Assay

Journal: bioRxiv

Article Title: Skeletal Metastasis of Prostate Cancer Is Augmented by Activation of EphA2 Noncanonical Signaling and Ligand-Deficient Bone Microenvironment

doi: 10.1101/2024.09.25.615079

Figure Lengend Snippet: A) Diagram depicting the serial propagation of TRAMP-C1 cells in syngeneic mice to select for cells with increased tumorigenic capacity. B) The in vivo selection of cells resulted in accelerated tumor growth and reduced latency. C) Immunoblot analyses of the in vivo selected (IV1) and the parental TRAMP-C1 cells using the indicated antibodies. D,E,F,G,H,I,J) Quantification of relative band intensity (n = 4). All bands normalized to tubulin, except for pY588 and pS897 which are normalized to total EphA2 expression. K) Immunoblot of TRAMP-C1 parental and IV1 cells after stimulation with Ephrin-A1-Fc ligand for the indicated times. L,M) MPC3 and MycCaP cells were propagated in syngeneic C57Bl/6 and FVB mice, respectively. Lysates of the parental and the in vivo selected cells (IV1) were subject to immunoblot using the indicated antibodies.

Article Snippet: Other antibodies were purchased as follows: Rabbit monoclonal anti-GAPDH (Cell Signaling Technology #2118S), rabbit monoclonal anti-pS473-Akt (Cell Signaling Technology #4060), rabbit polyclonal anti-pErk1/pErk2 (Cell Signaling Technology #9101S), rabbit monoclonal anti-EphA2 (Cell Signaling Technology #6697S), rat monoclonal anti-EphA2-PE (R&D Systems FAB639P), rabbit monoclonal anti-AR (Abcam ab133273), rabbit polyclonal anti-pY772-EphA2 (Cell Signaling Technology #8244S), rabbit monoclonal anti-pY588-EphA2 (Cell Signaling Technology #12677S), mouse monoclonal anti-Tubulin (R&D Systems MAB9344) Goat anti-rabbit and goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and from Biorad (#1706515, and #1706516 respectively).

Techniques: In Vivo, Selection, Western Blot, Expressing

Journal: bioRxiv

Article Title: Skeletal Metastasis of Prostate Cancer Is Augmented by Activation of EphA2 Noncanonical Signaling and Ligand-Deficient Bone Microenvironment

doi: 10.1101/2024.09.25.615079

Figure Lengend Snippet: A) Immunohistochemical (IHC) analyses of tissue microarray (TMA) prepared from primary, lymph node metastatic (LN), and bone metastatic human PCa tissues from the Rapid Autopsy Program of the Pacific Northwest PCa SPORE spotted. B and C) The relative IHC staining strengths of EphA2 and pS897-EphA2 on TMA was scored using 0-3 scale (0, absent; 1, weak; 2, moderate; 3, strong) . Data consists of 27 bone metastatic samples, and 18 soft tissue samples including 10 lymph node, 5 liver, and 3 lung metastatic samples. Comparisons were made using an unpaired t-test (B, p = 0.0003 C, p < 0.0001) D) mRNA expression analysis of human PCa metastatic biopsies revealed that bone metastases have the highest EphA2 expression compared to other sites of disease dissemination. Data from 208 samples from the SU2C/PCF Dream Team. mRNA Expression z-scores relative to all samples (log FPKM Capture) accessed via cBioportal. E) Kaplan-Meier curve of patients with bone metastases according to the levels of EphA2 expression.

Article Snippet: Other antibodies were purchased as follows: Rabbit monoclonal anti-GAPDH (Cell Signaling Technology #2118S), rabbit monoclonal anti-pS473-Akt (Cell Signaling Technology #4060), rabbit polyclonal anti-pErk1/pErk2 (Cell Signaling Technology #9101S), rabbit monoclonal anti-EphA2 (Cell Signaling Technology #6697S), rat monoclonal anti-EphA2-PE (R&D Systems FAB639P), rabbit monoclonal anti-AR (Abcam ab133273), rabbit polyclonal anti-pY772-EphA2 (Cell Signaling Technology #8244S), rabbit monoclonal anti-pY588-EphA2 (Cell Signaling Technology #12677S), mouse monoclonal anti-Tubulin (R&D Systems MAB9344) Goat anti-rabbit and goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and from Biorad (#1706515, and #1706516 respectively).

Techniques: Immunohistochemical staining, Microarray, Immunohistochemistry, Expressing

Journal: bioRxiv

Article Title: Skeletal Metastasis of Prostate Cancer Is Augmented by Activation of EphA2 Noncanonical Signaling and Ligand-Deficient Bone Microenvironment

doi: 10.1101/2024.09.25.615079

Figure Lengend Snippet: A) Immunoblot analysis of PC3 cells transduced with lentivirus to express WT-EphA2 or S897A-EphA2. Empty vector-transduced cells were used as control. Cells were starved for 24 hours in serum-free medium and stimulated with either FBS alone or FBS togethr with ephrin-A1-Fc. B) PC3 cells overexpressing WT-EphA2 or S897A-EphA2 mutant were xenografted subcutaneously into nude mice (n = 10 per group). Vector alone cells were used as control. Comparison between WT-EphA2 and S897A-EphA2 made with an unpaired t-test at day 46 post-injection (p = 0.0402).

Article Snippet: Other antibodies were purchased as follows: Rabbit monoclonal anti-GAPDH (Cell Signaling Technology #2118S), rabbit monoclonal anti-pS473-Akt (Cell Signaling Technology #4060), rabbit polyclonal anti-pErk1/pErk2 (Cell Signaling Technology #9101S), rabbit monoclonal anti-EphA2 (Cell Signaling Technology #6697S), rat monoclonal anti-EphA2-PE (R&D Systems FAB639P), rabbit monoclonal anti-AR (Abcam ab133273), rabbit polyclonal anti-pY772-EphA2 (Cell Signaling Technology #8244S), rabbit monoclonal anti-pY588-EphA2 (Cell Signaling Technology #12677S), mouse monoclonal anti-Tubulin (R&D Systems MAB9344) Goat anti-rabbit and goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and from Biorad (#1706515, and #1706516 respectively).

Techniques: Western Blot, Transduction, Plasmid Preparation, Control, Mutagenesis, Comparison, Injection

Journal: bioRxiv

Article Title: Skeletal Metastasis of Prostate Cancer Is Augmented by Activation of EphA2 Noncanonical Signaling and Ligand-Deficient Bone Microenvironment

doi: 10.1101/2024.09.25.615079

Figure Lengend Snippet: A) Analysis of log 2 transformed FPKM gene expression values extracted from bulk RNA seq published previously (Smith R. et al. Scientific Reports 2020 ) showing low Efna1-5 gene expression in commercially available castration resistant and androgen sensitive human PCa cell lines. B) Immunoblot characterization of select PCa cell lines reveals simultaneous high EphA2 expression and low EphrinA1 expression in metastatic castration resistant cells compared with androgen sensitive cells. C ) Quantitative analysis of data in (B). D) Kaplan-Meier survival curve from the MSK dataset ( Cancer Cell 2010 ). Samples were aggregated into the top 25 percent EFNA1 expressing ( EFNA1 High), and lower 75 percent EFNA1 expressing ( EFNA1 Low). Data were accessed via cBioportal.

Article Snippet: Other antibodies were purchased as follows: Rabbit monoclonal anti-GAPDH (Cell Signaling Technology #2118S), rabbit monoclonal anti-pS473-Akt (Cell Signaling Technology #4060), rabbit polyclonal anti-pErk1/pErk2 (Cell Signaling Technology #9101S), rabbit monoclonal anti-EphA2 (Cell Signaling Technology #6697S), rat monoclonal anti-EphA2-PE (R&D Systems FAB639P), rabbit monoclonal anti-AR (Abcam ab133273), rabbit polyclonal anti-pY772-EphA2 (Cell Signaling Technology #8244S), rabbit monoclonal anti-pY588-EphA2 (Cell Signaling Technology #12677S), mouse monoclonal anti-Tubulin (R&D Systems MAB9344) Goat anti-rabbit and goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and from Biorad (#1706515, and #1706516 respectively).

Techniques: Transformation Assay, Expressing, RNA Sequencing Assay, Western Blot

Journal: bioRxiv

Article Title: Skeletal Metastasis of Prostate Cancer Is Augmented by Activation of EphA2 Noncanonical Signaling and Ligand-Deficient Bone Microenvironment

doi: 10.1101/2024.09.25.615079

Figure Lengend Snippet: A) Lysates of PC-3 cells transduced by lentiviral vector expressing EFNA1 (EA1) or empty control vector were subject to immunoblot with the indicated antibodies. B,C) Quantification of pY588 marking canonical signaling and pS897 marking noncanonical signaling by EphA2, relative to total EphA2 expression. D) Vec. and EA1-expressing PC-3 cells were stimulated with recombinant exogenous EA1-Fc ligand in vitro for the indicated times, and lysates were subject to immunoblot with the indicated antibodies. E) PY99, a monoclonal antibody that recognizes most pY motifs, was used to probe overall pY profiles using lysates from Vec. and EA1-expressing PC-3 cells stimulated with EA1-Fc. N-cadherin and pY416-Src were also probed. F) PC-3 cells expressing EA1 exhibit reduced cell growth in an in vitro cell proliferation assay. G) Vec. control and EA1-expressing cells were implanted subcutaneously into nude mice. Paired t-test to be used to monitor (p = 0.0319). G) Bioluminescence imaging of mice four and six weeks after intratibial injection of PC3-vec. or PC-3-EA1 expressing cells tagged with luciferase. H) X-ray imaging of mice 6 weeks post intratibial injection of PC3-Vec and PC3-EA1 cells.

Article Snippet: Other antibodies were purchased as follows: Rabbit monoclonal anti-GAPDH (Cell Signaling Technology #2118S), rabbit monoclonal anti-pS473-Akt (Cell Signaling Technology #4060), rabbit polyclonal anti-pErk1/pErk2 (Cell Signaling Technology #9101S), rabbit monoclonal anti-EphA2 (Cell Signaling Technology #6697S), rat monoclonal anti-EphA2-PE (R&D Systems FAB639P), rabbit monoclonal anti-AR (Abcam ab133273), rabbit polyclonal anti-pY772-EphA2 (Cell Signaling Technology #8244S), rabbit monoclonal anti-pY588-EphA2 (Cell Signaling Technology #12677S), mouse monoclonal anti-Tubulin (R&D Systems MAB9344) Goat anti-rabbit and goat anti-mouse secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and from Biorad (#1706515, and #1706516 respectively).

Techniques: Plasmid Preparation, Expressing, Control, Western Blot, Recombinant, In Vitro, Proliferation Assay, Imaging, Injection, Luciferase

Journal: Biology of Reproduction

Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells ^†

doi: 10.1093/biolre/ioz156

Figure Lengend Snippet: Analysis of EPHA2 expression in spermatogonia. (A, B) RT-PCR analyses of Eph family genes in CDH1-selected testis cells (A) and GS cells (B). (C) Double immunostaining of 7-day-old pup and 6-week-old adult mouse testes using antibodies against EPHA2 and GFRA1, CDH1, or KIT. At least 67 cells (pup) or 52 cells (adult) in 10 fields were counted for each antigen. Arrows and arrowheads indicate cells expressing GFRA1 and CDH1, respectively. (D) Immunostaining of adult human testis using antibodies against EPHA2 and GFRA1. (E) Immunostaining of adult mouse testis using antibodies against EFNA1 and GATA4. Bar = 20 μm (C–E). Asterisk indicates statistical significance (P < 0.05).

Article Snippet: For MACS, dissociated testis cells (2 × 10 7 for wild-type and green mice; 5–10 × 10 7 for ROSA26 mice) were incubated with anti-EPHA2 antibody (5 μg/ml; MAB639; R&D systems, Minneapolis, MN) or anti-cadherin 1 antibody (5 μg/ml; ECCD2, gift from Dr. Masatoshi Takeichi from RIKEN CDB, Kobe, Japan) for 10 min at 4 °C.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Double Immunostaining, Immunostaining

Journal: Biology of Reproduction

Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells ^†

doi: 10.1093/biolre/ioz156

Figure Lengend Snippet: Enrichment of mouse SSCs from the adult testis by MACS or FACS. (A) Cell recovery after MACS using anti-CDH1 or anti-EPHA2 antibodies (n = 3). (B) Macroscopic appearance of the recipient testis showing colonization of green mouse testis cells selected by MACS using an anti-EPHA2 antibody. (C) Colony count (n = 18 for EPHA2; n = 16 for control). (D) Fractionation of CDH1-selected ROSA mouse testis cells according to EPHA2 expression levels. Cells in the spermatogonia gate were divided into three populations (EPHA2negative, EPHA2low, and EPHA2high). (E) Real-time PCR analysis of spermatogonia genes (n = 3). (F) Macroscopic appearance of recipient testis transplanted with the EPHA2high population. The three populations of cells were transplanted into the seminiferous tubules of busulfan-treated mouse testes. (G) Colony count (n = 12). (H) Histological appearance of recipient testis showing normal spermatogenesis. Bar = 1 mm (B, F), 10 μm (H). Asterisk indicates statistical significance (P < 0.05).

Article Snippet: For MACS, dissociated testis cells (2 × 10 7 for wild-type and green mice; 5–10 × 10 7 for ROSA26 mice) were incubated with anti-EPHA2 antibody (5 μg/ml; MAB639; R&D systems, Minneapolis, MN) or anti-cadherin 1 antibody (5 μg/ml; ECCD2, gift from Dr. Masatoshi Takeichi from RIKEN CDB, Kobe, Japan) for 10 min at 4 °C.

Techniques: Cell Recovery, Control, Fractionation, Expressing, Real-time Polymerase Chain Reaction

Journal: Biology of Reproduction

Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells ^†

doi: 10.1093/biolre/ioz156

Figure Lengend Snippet: Impaired proliferation and increased apoptosis by Epha2 KD in GS cells. (A) Flow cytometric analysis of EPHA2 expression after cytokine (FGF2 and GDNF) supplementation. Cells were cultured without cytokines for 2 days (NF) and then restimulated with FGF2 and GDNF (FG). Cells were analyzed on the next day after cytokine stimulation (n = 3). (B) Flow cytometric analysis of EPHA2 expression of GS cells 5 days after Epha2 KD (n = 3). (C) Cell recovery after Epha2 KD on MEFs. Cells were recovered 6 days after transfection (n = 12). (D) Cell recovery after Epha2 KD on laminin-coated plates (n = 9). GS cells were transfected with shRNA against Epha2, and cells were plated on laminin 2 days after transfection. Cells were recovered 2.5 h after plating. The relative cell recovery from the initially plated cells is indicated. (E) Flow cytometric analysis of ITGA6 expression after Epha2 KD. Fluorescence intensity was determined on the next day after transfection (n = 3). (F) Quantification of proliferating cells by MKI67 staining with at least 528 cells in 14 fields counted. Cells were recovered 4 days after transfection. (G) Quantification of apoptotic cells by TUNEL staining. TUNEL-stained cells were counterstained with Hoechst 33342 with at least 636 cells were counted. Cells were recovered 4 days after transfection. Scramble shRNA was used as a control in all KD experiments Bar = 50 μm (F, G). Asterisk indicates statistical significance (P < 0.05).

Article Snippet: For MACS, dissociated testis cells (2 × 10 7 for wild-type and green mice; 5–10 × 10 7 for ROSA26 mice) were incubated with anti-EPHA2 antibody (5 μg/ml; MAB639; R&D systems, Minneapolis, MN) or anti-cadherin 1 antibody (5 μg/ml; ECCD2, gift from Dr. Masatoshi Takeichi from RIKEN CDB, Kobe, Japan) for 10 min at 4 °C.

Techniques: Expressing, Cell Culture, Cell Recovery, Transfection, shRNA, Fluorescence, Staining, TUNEL Assay, Control

Journal: Biology of Reproduction

Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells ^†

doi: 10.1093/biolre/ioz156

Figure Lengend Snippet: Analysis of EPHA2 and self-renewal factor receptors after Epha2 KD. (A) Western blot analysis of GS cells after Epha2 KD (n = 3). Cells were recovered 5–6 days after transfection. (B) Western blot analysis of GS cells after cytokine stimulation. Cells were cultured without cytokines for 2 days, and samples were collected on the next day after restimulation with FGF2 and GDNF. (C–E) Immunoprecipitation using antibodies against EPHA2 (C), RET (D), or FGFR2 (E).

Article Snippet: For MACS, dissociated testis cells (2 × 10 7 for wild-type and green mice; 5–10 × 10 7 for ROSA26 mice) were incubated with anti-EPHA2 antibody (5 μg/ml; MAB639; R&D systems, Minneapolis, MN) or anti-cadherin 1 antibody (5 μg/ml; ECCD2, gift from Dr. Masatoshi Takeichi from RIKEN CDB, Kobe, Japan) for 10 min at 4 °C.

Techniques: Western Blot, Transfection, Cell Culture, Immunoprecipitation

Journal: Biology of Reproduction

Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells ^†

doi: 10.1093/biolre/ioz156

Figure Lengend Snippet: Functional analysis of Epha2 by spermatogonial transplantation. (A) Real-time PCR analysis of spermatogonial markers after Epha2 KD (n = 3). Cells were recovered 4 days after transfection, and the relative expression levels were determined by measuring the ratio between the experimental shRNA and control shRNA (indicated by the horizontal bar). (B) Cell recovery after transfection (n = 3). Cells were collected 2 days after transfection. (C) Real-time PCR analysis of Epha2 expression using GS cells (n = 3). GS cells were transfected by lentiviruses that express shRNA against Epha2. Cells were recovered on the next day after transfection. (D) Macroscopic appearance of recipient testis transplanted with pup testis cells after Epha2 KD or Epha2 CA OE. (E) Colony count (n = 17 for Epha2 KD; n = 18 for Epha2 CA). (F, G) Immuno- (F) or lectin (G) staining of recipient testes. Bar = 1 mm (D), 20 μm (F, G). Asterisk indicates statistical significance (P < 0.05).

Article Snippet: For MACS, dissociated testis cells (2 × 10 7 for wild-type and green mice; 5–10 × 10 7 for ROSA26 mice) were incubated with anti-EPHA2 antibody (5 μg/ml; MAB639; R&D systems, Minneapolis, MN) or anti-cadherin 1 antibody (5 μg/ml; ECCD2, gift from Dr. Masatoshi Takeichi from RIKEN CDB, Kobe, Japan) for 10 min at 4 °C.

Techniques: Functional Assay, Transplantation Assay, Real-time Polymerase Chain Reaction, Transfection, Expressing, shRNA, Control, Cell Recovery, Staining

Journal: Cell Adhesion & Migration

Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

doi: 10.1080/19336918.2015.1107693

Figure Lengend Snippet: Expression of endogenous and exogenous/dominant negative EphA2 in U937, EphA2ΔC-EGFP-U937, J774.1, and EphA2ΔC-EGFP-J774.1 cells. ( A ) Typical phase contrast and fluorescence micrographs highlighting the expression of the EphA2ΔC-EGFP protein. ( B ) EphA2 mRNAs amplified from the intracellular and extracellular regions by RT-PCR showing the expression of endogenous and total (endogenous plus exogenous) EphA2, respectively, in U937 and its subline. Densitometric quantification of the RT-PCR amplification levels of EphA2 from 3 independent experiments normalized to the levels of GAPDH. Data is presented as the mean ± SD. ** P < 0.01. ( C ) Amplification of endogenous and exogenous EphA2 in J774.1 cells and their subline cells by RT-PCR. Densitometric quantification of the RT-PCR amplification levels were determined from 3 independent experiments and normalized to the levels of GAPDH. Data is presented as the mean ± SD.

Article Snippet: The rat monoclonal PE-conjugated antibody against mouse EphA2 used in this study was from R&D Systems (233720).

Techniques: Expressing, Dominant Negative Mutation, Fluorescence, Amplification, Reverse Transcription Polymerase Chain Reaction

Journal: Cell Adhesion & Migration

Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

doi: 10.1080/19336918.2015.1107693

Figure Lengend Snippet: Representative histograms showing EphA2 and/or EGFP expression in U937 and EphA2ΔC-EGFP-U937 cells ( A ) as well as J774.1 and EphA2ΔC-EGFP-J774.1 cells ( B ).

Article Snippet: The rat monoclonal PE-conjugated antibody against mouse EphA2 used in this study was from R&D Systems (233720).

Techniques: Expressing

Journal: Cell Adhesion & Migration

Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

doi: 10.1080/19336918.2015.1107693

Figure Lengend Snippet: Molecular association of EphA2 with the β2 integrin/ICAM1 ( A ) and β2 integrin/VCAM1 complexes ( B ) in U937 and EphA2ΔC-EGFP-U937 cells.

Article Snippet: The rat monoclonal PE-conjugated antibody against mouse EphA2 used in this study was from R&D Systems (233720).

Techniques:

Journal: Cell Adhesion & Migration

Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

doi: 10.1080/19336918.2015.1107693

Figure Lengend Snippet: Primers and cycle numbers for PCR amplification used in U937 cells

Article Snippet: The rat monoclonal PE-conjugated antibody against mouse EphA2 used in this study was from R&D Systems (233720).

Techniques: Amplification

Journal: Cell Adhesion & Migration

Article Title: EphA2 promotes cell adhesion and spreading of monocyte and monocyte/macrophage cell lines on integrin ligand-coated surfaces

doi: 10.1080/19336918.2015.1107693

Figure Lengend Snippet: Primers and cycle numbers for PCR amplification used in J774.1 cells

Article Snippet: The rat monoclonal PE-conjugated antibody against mouse EphA2 used in this study was from R&D Systems (233720).

Techniques: Amplification